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Glutathione can not only be used in drugs, but also as a base material for functional foods

Diet While NAC itself is not naturally present in meaningful amounts in food, it is derived from the amino acid cysteine.30 Consuming adequate protein helps provide the building blocks your body uses to produce glutathione and support its natural antioxidant defenses.6 Foods rich in cysteine include:5 Poultry Eggs Beef and pork Fish and seafood Dairy products Beans and lentils Soy foods Although dietary cysteine is not a direct replacement for NAC supplementation, maintaining adequate protein intake can help support many of the same biological pathways that make NAC an area of interest for researchers

As with other glutathione peroxidases, GPx1 has a conserved catalytic tetrad composed of Sec or Cys, Gln, Trp, and Asn, where the Sec is surrounded by four arginines (R 57, 103, 184, 185

Also, if re-naturing the protein from inclusion bodies, be sure that all denaturants have been removed from the buffer, either through exhaustive dialysis or application of the sample to a desalting column, before applying to the glutathione column