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Following the injection, imaging was performed using an IVIS Spectrum system (PerkinElmer) at various time points: 1 h, 4 h, 7 h, 12 h, 24 h, 36 h, 48 h, and 60 h post-injection, with an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm

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30 As a canonical byproduct of glucose metabolism, it is primarily generated through glycolysis, where glucose is converted to pyruvate and then to lactate by lactate dehydrogenase (LDH)