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Cells were maintained in a humidified environment of 5% CO 2 at 37 C, cultured until an optical confluence of 75-100%, detached with 0.05% trypsin-EDTA 1 (25300-054, Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA), at 37 C for approximately 5 min, and split to new plates according to the experimental procedures

monnieri , and garlic-derived allicin, contribute to enhanced neuroprotection and reduced A burden, thereby supporting cognitive health ( 3.1 Addressing unresolved challenges and bridging research gaps in human studies on herbal treatments for Alzheimers disease Clinical trials highlight the therapeutic potential of natural products in AD patients, demonstrating improvements in memory, cognitive function, and neuropsychiatric symptoms

Splitting into morning and evening doses helps maintain consistent levels