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After deparaffinization, antigen retrieval and permeabilization, the samples were blocked and probed with the rabbit polyclonal antibodies against loricrin, involucrin (both Abcam, UK), filaggrin, KLK7 and TG1 (all three Invitrogen, USA) and appropriate anti-rabbit secondary antibody conjugated with Alexa Fluor 555 (Invitrogen, USA)

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Among them are iron, magnesium, sodium, zinc, fluorine, and many other elements from the periodic table (excluding carbon)