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Identification of causative factors is essential not only for clinical management and genetic counseling, but also for implementing fertility preservation strategies in high-risk individuals

cDNA was then used as template for RT-qPCR with a QuantStudio 3 Real-Time PCR system (Applied Biosystems) using PowerUP SYBR Green Master Mix (Applied Biosystems) and 500 nM of target gene specific forward and reverse primers, using the recommended fast cycling mode

Ongoing research continues to expand understanding of how glutathione functions across different clinical contexts, and providers can expect continued refinement in how this molecule is discussed and applied in practice

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