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This result was confirmed statistically using a non-parametric analysis of variance (KruskallWallis test), followed by post-hoc analysis (Wilcoxon method) that showed the procedural blank froze at a significantly different temperature than all of the compounds (Supplementary Table 2)

Cell Metab 24:566581 Feoktistova M, Geserick P, Leverkus M (2016) Crystal violet assay for determining viability of cultured cells

The increased value of fluorescence intensity (ex 470/em 530 nm) indicates lipid-derived radicals

Storage is critical: youll keep the vial refrigerated and protected from light (see Storage guidelines above)