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Passage 1 or 2 cultures were seeded onto collagen IV (C7521, Sigma, St Louis, MO) coated 12 mm Transwell inserts (3460, Corning Costar Corporation, Cambridge, MA) and grown in ALI media to 80% confluence before exposing the apical surface to air

Lead scientist Dr Viktoria Schwarz, from the University of Saarland in Homburg, Germany, said: We found evidence that pre-consumption of red wine prevented most of the vascular injury caused by smoking. She added that since the study focused on young, healthy non-smokers it was not clear whether the findings would apply to the elderly, sick, or habitual smokers