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Classical acyltransferases (KAT family) and deacylases (HDAC and SIRT family) are believed to catalyze all lactylation modifications and delactylation processes, but several non-classical acyltransferases are also included, such as AARS1, AARS2 (Fig

the software can be downloaded from the following website: Bioinformatics methods predict lactylation of Lys447 in USP2 A DeepKla online tool ( was used to predict which Lys site in USP2 could be lactylated, with low score values suggesting better results

As a widely used healthcare guideline for opened multi-dose vials, vials are commonly dated and discarded within 28 days after first puncture unless the manufacturer states a different timeframe