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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

Though functional roles of the VGF peptides detected here remain to be established, consistent changes in VGF abundance across proteoforms between diagnostic classes in the present study, as well as previous literature, suggest clear roles of VGF peptides in cognitive functioning 15

Monitor Blood Counts: Regular monitoring of hemoglobin and B12 levels is recommended, especially for anemia

Baseline scores for each dimension are shown for the two treatment groups with respect to the population norm of patients with chronic disease from the Medical Outcomes Study (31) in Figure 5