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Machine learning models, including gradient boosted trees and logistic regression, have been employed to predict the presence of spermatozoa in testicular biopsies of patients with non-obstructive azoospermia (NOA)

The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

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