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They permit different levels of study: organ, tissue, cell (one or several populations), they allow large screening of effects with a very small amount of test material and are very well adapted for the study of mechanisms, mainly for short term studies [17, 20, 21]

The film follows John McClane, a no-nonsense New York cop, as he arrives in California on Christmas Eve to attend the work party of his wife, Holly, who has been living apart from him, in an attempt to reconcile the relationship

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It put things in perspective in a really deep way,' he told the Today show last year, while discussing the highly-acclaimed film