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Vitamin B12 deficiency left untreated can lead to pernicious anaemia or neurological problems

doi: 10.3390/md10112596

Dose and frequency matter: Because injections can deliver more peptide, you might not dose every night depending on the protocol, it might be once daily or every other day

After incubation, the coverslip was transferred into the original medium for 15 min and for labeling, neurons were three times washed with 1x PBS and fixed with 4 % PFA/ 4 % sucrose in PBS for 15 min at room temperature (RT)