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Finally, as both increasing age and germ-free conditions have been associated with a decline Drosophila antioxidant responses 22,23 , we defined the requirement of the antioxidant response system in our model and find, in agreement with vertebrate studies, that genetic and pharmacologic manipulations of antioxidant response pathways results in altered sensitivity to APAP toxicity 24,25

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A 29 gauge needle produces significantly less discomfort than a 25 gauge needle and is thin enough that most patients describe the sensation as a mild pinch rather than a sting