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Heterogeneity was assessed across the studies using the I 2 statistic, with 25, 50, and 75% denoting low, moderate, and high heterogeneity, respectively 27

HPLC and mass spectrometry were carried out following previous methods, with modifications ( Monoamine oxidase A activity, qRT-pCR and dopamine content ELISA kit The striatum of mice was excised with a mold in a buffer solution at 4C, quickly frozen in liquid nitrogen, and stored at 80C

Of course you can use your own judgement as to how much paper you pull out, mostly determined by what sized roller you are using, but having a nice clean cut to work with makes an huge difference