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Both live/dead staining with DAPI and bead-based normalization were employed to ensure accurate quantification of absolute cell numbers per colon

Glutathione, or GSH, helps to prevent damage to our cells by reactive oxygen species like free radicals and peroxides

Small and unlikely to affect function, His-tagged proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column

Additionally, regulating other pathways related to oxidative stress, such as the SIRT1/PGC-1 mitochondrial biosynthesis pathway and the AMPK energy sensing pathway, can also indirectly improve the oxidative-reductive state of cells [246]