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Methods for preparing a total nonparenchymal cell fraction are described in Application Sheet C26 Section 2 describes the options for sedimentation on to a density barrier Section 3 describes the two-layer gradient Section 4 describes the flotation strategy Section 5 contains important notes to Sections 2, 3 and 4 Section 6 briefly describes some of the methodological variations reported in recent papers 2

Mukherjee KK, Lee AY, Zhu L, Darvas M, & Ladiges W

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