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The in vivo environment is considerably more complex, involving intricate biological networks and an active immune system, which are not present in in vitro cultures

Furthermore, unlike other known lipases, adiponutrin expression is dramatically upregulated in animals that have been refed following a fast, whereas adiponutrin mRNA is almost undetectable in fasted animals (9)

Animal studies show fat loss effects, but human results have been mixed

While alpha diversity measures did not significantly differ between groups, beta diversity principal coordinates analyses suggested that mice from the same treatment group were the most similar