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Description

T., & Tain, Y

This region is responsible for the actinbinding and cellmigration properties of the full thymosin molecule

The supernatant was applied to an Ni-nitroloacetic acid affinity column (Qiagen, Hilden, Germany) pre-equilibrated with lysis buffer and then washed with wash buffer comprising 50 mM sodium phosphate, 300 mM NaCl, and 20 mM imidazole (pH 8.0)

On day 9 following acquisition training, an additional trial was conducted where the platform was removed and the rat was required to swim for the entire 120 second duration