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The overactivation of microglia leads to the release of pro-inflammatory factors such as Interleukin-1 beta (IL-1), Tumor Necrosis Factor-alpha (TNF- ), and Interleukin-6 (IL-6), which directly damage dopaminergic neurons in the substantia nigra pars compacta (SNpc) (McCarty and Lerner, 2020)

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Then, a 24-h hypoxia intervention was also conducted on adherent human myeloid leukemia mononuclear cells (THP-1 cells) induced by Phorbol 12-Myristate 13-Acetate (PMA), a THP-1 cell differentiation inducer

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