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However, due to the complexity of the disease and the singleness of drug targets, it is difficult to develop excitotoxicity antagonists in clinical practice

Its molecular structure was optimized through genetic engineering technology: an additional 13 amino acids were added to the N-terminus of natural IGF-1 (70 amino acids, molecular weight 7.4 kDa) to form a long-chain structure of 83 amino acids (molecular weight 9.11 kDa), and the third glutamic acid (Glu) was replaced with arginine (Arg), hence the name "R3"

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We analyzed EVs using TEM and nano-FCM according to previously published protocols 20