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And upon further investigation, they came up with several possible explanations to understand the main cause of the negative results: Silencing in plant cells, which means the cell shuts down the promoter activity or degrades the RNA of the highly expressed genes to reduce the negative effects resulting from high expression High levels of lux genes are toxic to the plant cell An unidentified mutation in the promoter means none of those were working correctly Selectable marker Switcheroo So you remember how an easy way to tell if a plant has successfully incorporated a gene is if it doesnt die from the antibiotics because of the antibiotic-resistant reporter genes