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Multi-mechanism research designs

Contrary to the two models of halogenation in NRP biosynthesis presented above where halogenation occurs either on free amino acids or en route peptide assembly, chlorination during the biosynthesis of the RiPP antibiotic NAI-107 occurs after all other modifications on the RiPP precursor peptide have been installed, and the post-translationally modified C-terminal core has been proteolytically removed from the unmodified N-terminal core peptide [1, 42]

The results of several animal and clinical trials support these findings (24-26)