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[16] [121] Investigations into the intestinal absorption of B 12 confirm that the upper limit of absorption per single oral dose is about 1.5 g, with 50% efficiency

Common lab protocols involve reconstitution in bacteriostatic water and subcutaneous injection in animal models using calibrated syringes to achieve precise dosing

Vitamin B12 injections are also used by people with impaired vitamin B12 absorption or deficiency called Pernicious anaemia caused by: Inadequate dietary intake of B12 Loss of gastric parietal cells in chronic atrophic gastritis Structural damage or surgical resection of the small intestine or stomach Long-term use of acid regulating medication Alcoholism Gastric banding surgery Celiacs disease Pernicious anaemia Crohns disease Until now, injection of methyl-B12 has required a needle

In controlled laboratory settings, it is utilized to study the relationship between NNMT inhibition, NAD+ biosynthesis, and cellular energy metabolism