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The beneficial effects of the MD are hypothesized to stem from its anti-inflammatory and antioxidant properties, attributed to components such as extra virgin olive oil, vegetables, fruit, and long-chain omega-3 fatty acids, which may mitigate endometriosis risk and symptoms, whereas trans-fats and high red meat intake have been linked to increased risk

The cells were then washed twice with PBS and then overlaid with cystine-free RPMI supplemented with 20 nM Sytox Green (Fisher Scientific, S7020) and the indicated treatment

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