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After three PBS washes, cells were resuspended in cold PBS with 1% FBS and 50 g ml 1 Gen, with or without 2 M aTc, for 3 h

It is tempting to speculate that a previously described internal promoter of yet unknown function may play a role in adjusting and fine tuning of the independent transcription of cesA and cesB, thereby decoupling CesA and CesB to a certain extend 11