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The leak pathway allows macromolecules to cross the epithelial barrier and is regulated by the cytoskeleton and epithelial long myosin light chain kinase splice variant 1 (MLCK1)

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This fact is relevant as transgenic mice overexpressing IGFBP-2 were resistant to developing obesity when fed with high fat diet [174], suggesting that this molecule could have a direct effect on preadipocyte differentiation