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After a 24-h treatment, the samples of 100 L of the extracellular medium were taken from 500 L of the total culture well volume

Ototoxicity is defined as an increase in the auditory threshold by at least 20 dB at any one test frequency, or at least 10 dB at any two adjacent frequencies, or loss of response at three consecutive frequencies between the baseline and during follow-up studies

You can find more detail on B12 treatment timelines and schedules

The world market for riboflavin production for human and animal use has more than doubled in 13 years, from 4000 t a 1 in 2002 to 9000 t a 1 in 2015 (Schwechheimer et al., 2016)