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However, when performing a similar pH titration with the U597C variant minimal changes in the formation of the thiolate-FAD charge transfer are observed (Figure 9A) suggesting both that the 571 histidine residue is the acting general base and the C159 residue is highly shielded from solvent

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Gait patterns were monitored and assessed subsequent to the training every 15 minutes for 2 hours, while adding in secondary tasks of intensifying complexity levels, and covertly when subjects were inattentive to measurements being taken

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